@article{Almrasy_2018, title={VALIDATED SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF DORZOLAMIDE HYDROCHLORIDE IN ITS PURE FORM AND PHARMACEUTICAL FORMULATIONS VIA DERIVATIZATION WITH 4-CHLORO-7-NITROBENZO-2-OXA-1, 3-DIAZOLE (NBD-CL)}, url={https://innoriginal.com/index.php/iijs/article/view/161}, abstractNote={<strong>Objectives</strong>: An accurate and precise spectrofluorimetric method was developed and validated for the determination of dorzolamide hydrochloride in its pure form and pharmaceutical formulations. <strong>Method</strong>: Based on nucleophilic substitution reaction of target drug with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 541 nm after excitation at 404 nm. <strong>Results</strong>: Under optimum condition, the proposed method obeys Beer’s law in range (1- 8 µg/mL) and the reaction mechanisms were presented. <strong>Conclusion</strong>: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of the drug in its pure form and pharmaceutical formulations. The obtained results were statistically compared with those of the reported method and found to be in good agreement.}, journal={Innoriginal: International Journal of Sciences}, author={Almrasy, Ahmed Ali}, year={2018}, month={Jun.}, pages={17–20} }