Sanative effect of a low-cost Novel Green Formulation – IM-SSS20 to minimize the inflammatory and cytokine storm against Respiratory Diseases

Sangita Agarwal, Soumendra Darbar, Srimoyee Saha, Tathagata Deb

Abstract


Objective: To develop a herbal formulation combining four ingredients namely Ocimum sanctum, Green tea, Nyctanthes arbor-tristis and  Hygrophila auriculata  to combat infections that affect the respiratory system leading to inflammation, stress and other complications.

Method: The management of respiratory conditions was tried with the low cost multi herbal preparation IM-SSS 20.We have developed a herbal formulation combining four ingredients namely Ocimum sanctum, Green tea, Nyctanthes arbor-tristis  and  Hygrophila auriculata. Plant secondary metabolites in the developed multi-herbal formulation (MHF) (IM-SSS-20) were detected through quantitative analysis. Anti-bacterial and anti-fungal activity of the IM-SSS-20 were also determined. In-vitro clinical study was done to measure the haematological parameters like Haemoglobin (Hb), Haematocrit (PCV), Total RBC (RBC) and Total WBC (TC)  and estimate the expression of several biomarkers like IL-6, IL-8, IL-10 and TNF-α.

Results:The phytochemicals like triterpenes, flavonoids, saponins, and tannins were abundantly found in the IMSSS-20 extract. The inhibitory action of IM-SSS20 was dose-dependent and significant anti-bacterial activity was seen in the case of Escherichia coli followed by Streptococcus aureus and Klebsiella pneumonia respectively. The IM-SSS 20 also showed antifungal activity, the activity was maximum in case of C. albicans and was least for A.niger. In this study we observed a lower value of hemoglobin, hematocrit, total RBC and total WBC in respiratory infected patients. IM-SSS20 was effective in normalizing the concentration of Hb, PCV, RBC and TC. 

Conclusion: The multi-herbal formulation IM-SSS20 showed promising results in-vitro studies and further studies are required to explore its efficacy in humans. This low-cost formulation can improve health as well as economy of the developing countries.

KEYWORDS: Respiratory diseases, Ocimum sanctum, Green tea, Nyctanthes arbor-tristis  and  Hygrophila auriculata.


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Innoriginal: International Journal of Sciences (ISSN: 2349-7041)

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