PHYTOCHEMICAL COMPOSITION AND LARVICIDAL ACTIVITY OF ESSENTIAL OIL FROM THE LEAVES OF PLEIOSPERMIUM ALATUM (WALL. EX WT. & ARN) SWINGLE (RUTACEAE) AGAINST AEDES AEGYPTI, ANOPHELES STEPHENSI AND CULEX QUINQUEFASCIATUS (DIPTERA: CULICIDAE)
AbstractÂ Â Â Â Â Â Â Â The present study was conducted to evaluate the essential oil from the leaves of Pleiospermium alatum (Wall. ex Wt. & Arn) Swingle (Rutaceae) was evaluated for mosquito larvicidal activity against the larvae of Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. GC and GC- MS analyses revealed that the essential oil contain fourteen compounds. D-Limonene (34.02%) was identified as a major chemical compounds followed by Elemol (18.04%) and Î³â€“Terpinene (11.83%). The larval mortality was observed after 12 and 24 hours of exposure periods. The results revealed that essential oil potential larvicidal activity against the 4th instar larvae of A. aegypti, A. stephensi and C. quinquefasciatus. After 12 h of exposure period the larvicidal activities were LC50=95.2 and LC90=366.7Â ppm (A. aegypti), LC50=72.6 and LC90=305.2Â ppm (A.stephensi) and LC50=104.4 and LC90=373.6Â ppm (C. quinquefasciatus) and the larvicidal activities after 24Â h of exposure period was LC50=53.8 and LC90=251.0Â ppm (A.aegypti), LC50=44.4 and LC90=213.8Â ppm (A. stephensi), and LC50=143.3 and LC90=375.5Â ppm (C. quinquefasciatus). The present study showed the leaves of the essential oil from the P. alatum and its D-Limonene major compound may have potential for use in control of A. aegypti, A. stephensi and C. quinquefasciatus and could be useful in search of new, safe, inexpensive and more effective control of vector mosquitoes. Key Words: Pleiospermium alatum. GC-MS. Aedes aegypti. Anopheles stephensi. Culex quinquefasciatus
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