Ethanol Intoxicated Hepatic Oxidative Stress Mitigated by Poly-Herbal Formulation – Trasina® in Murine Model
AbstractBackground: The main focus and aim of this scientific novel work was to find out the probable ameliorative effect of poly-herbal formulation (Trasina®) on serum and liver antioxidant enzymes activities in ethanol intoxicated organ dysfunctions in mice model. Methods: Forty Swiss albino adult male mice were taken from our animal house and randomly break into 4 groups; Group-1 served as control, Group-2 orally treated with ethanol (50% v/v), Group-3 pre-treated with herbal medicine (Trasina®) along with ethanol (50% v/v), and Group-4 only treated with poly-herbal formulation (Trasina®) without ethanol daily. Completion of six weeks treatment the animals were euthanized and livers were removed immediately and used fresh or kept frozen until analysis. Blood was taken from the animals before sacrifice for measurement the antioxidant parameters first and second order enzymes i.e. catalase (CAT), super oxide dismutase (SOD), glutathione –S transferees (GST) and reduce glutathione (GSH) from sera. Results: Activities of all antioxidant enzymes i.e. SOD, CAT, GSH and GST in serum and liver were significantly decreased in the ethanol intoxicated mice than in the controls. Treatment with herbal medicine (Trasina®) upon ethanol intoxication significant elevated the all antioxidant activities serum and liver. Conclusions: Results obtained from the present study clearly predict that treatment with poly-herbal formulation (Trasina®) might be a potent antioxidant that exerts beneficial effects on both catalase (CAT), super oxide dismutase (SOD), glutathione –S transferees (GST) and glutathione peroxidase (GPx) activities in ethanol intoxicated mice and inhibit organ damage.
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