Ethanol Intoxicated Hepatic Oxidative Stress Mitigated by Poly-Herbal Formulation – Trasina® in Murine Model


  • Soumendra Darbar 1Research and Development Division, Dey’s Medical Stores (Mfg.) Ltd., 62, Bondel Road, Kolkata-700019, West Bengal, India
  • Srimoyee Saha Department of Physics, Jadavpur University, 188, Raja S C Mallick Road, Kolkata-700032, West Bengal, India
  • Atiskumar Chatopadhyay Principal Secretary, Faculty Council of Science, Jadavpur University, 188, Raja S C Mallick Road, Kolkata-700032, West Bengal, India


Background: The main focus and aim of this scientific novel work was to find out the probable ameliorative effect of poly-herbal formulation (Trasina®) on serum and liver antioxidant enzymes activities in ethanol intoxicated organ dysfunctions in mice model.   Methods: Forty Swiss albino adult male mice were taken from our animal house and randomly break into 4 groups; Group-1 served as control, Group-2 orally treated with ethanol (50% v/v), Group-3 pre-treated with herbal medicine (Trasina®) along with ethanol (50% v/v), and Group-4 only treated with poly-herbal formulation (Trasina®) without ethanol daily. Completion of six weeks treatment the animals were euthanized and livers were removed immediately and used fresh or kept frozen until analysis. Blood was taken from the animals before sacrifice for measurement the antioxidant parameters first and second order enzymes i.e. catalase (CAT), super oxide dismutase (SOD), glutathione –S transferees (GST) and reduce glutathione (GSH) from sera.   Results: Activities of all antioxidant enzymes i.e. SOD, CAT, GSH and GST in serum and liver were significantly decreased in the ethanol intoxicated mice than in the controls. Treatment with herbal medicine (Trasina®) upon ethanol intoxication significant elevated the all antioxidant activities serum and liver.   Conclusions: Results obtained from the present study clearly predict that treatment with poly-herbal formulation (Trasina®) might be a potent antioxidant that exerts beneficial effects on both catalase (CAT), super oxide dismutase (SOD), glutathione –S transferees (GST) and glutathione peroxidase (GPx) activities in ethanol intoxicated mice and inhibit organ damage.

Author Biographies

Soumendra Darbar, 1Research and Development Division, Dey’s Medical Stores (Mfg.) Ltd., 62, Bondel Road, Kolkata-700019, West Bengal, India

Head Research and Development Division

Srimoyee Saha, Department of Physics, Jadavpur University, 188, Raja S C Mallick Road, Kolkata-700032, West Bengal, India

Research Scientist, Department of Physics

Atiskumar Chatopadhyay, Principal Secretary, Faculty Council of Science, Jadavpur University, 188, Raja S C Mallick Road, Kolkata-700032, West Bengal, India

Principal Secretary, Faculty Council of Science, Jadavpur University


Sies H, Jones DP. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nature Reviews Molecular Cell Biology. 2020; 21(7):363-383.

Huihui Z, Xin L, Zisong X, Yue W, Zhiyuan T, Meijun A, Yuehui Z, Wenxu Z, Nan X, Guangyu S. Toxic effects of heavy metals Pb and Cd on mulberry (Morus alba L.) seedling leaves: Photosynthetic function and reactive oxygen species (ROS) metabolism responses. Ecotoxicology and environmental safety. 2020; 195:110469.

Zorov DB, Juhaszova M, Sollott SJ. Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiological reviews. 2014; 94(3):909-950.

Yu Z, Li Q, Wang J, Yu Y, Wang Y, Zhou Q, Li P. Reactive oxygen species-related nanoparticle toxicity in the biomedical field. Nanoscale research letters. 2020; 15:1-4.

Goc Z, Kapusta E, Formicki G, Martiniaková M, Omelka R. Effect of taurine on ethanol‑induced oxidative stress in mouse liver and kidney. Chin J Physiol. 2019; 62:148-156.

Jing L, Jin CM, Li SS, Zhang FM, Yuan L, Li WM, et al. Chronic alcohol intake‑induced oxidative stress and apoptosis: Role of CYP2E1and calpain‑1 in alcoholic cardiomyopathy. Mol Cell Biochem. 2012; 359:283‑292.

Simplicio JA, do Vale GT, Gonzaga NA, Leite LN, Hipólito UV, Pereira CA, et al. Reactive oxygen species derived from NADPH oxidase play a role on ethanol‑induced hypertension and endothelial dysfunction in rat resistance arteries. J Physiol Biochem. 2017; 73:5‑16.

Darbar S, Chakraborty MR, Chattopadhyay SP, Ghosh, B. Protective effect of Livina, a polyherbal liquid formulation against ethanol induced liver damage in rats. Ant sci life. 2009; 28(3):14-17.

Khodaei F, Kholghipour H, Hosseinzadeh M, Rashedinia M. Effect of sodium benzoate on liver and kidney lipid peroxidation and antioxidant enzymes in mice. J Rep Pharm Sci. 2019; 8:217-223.

EL-Shenawy NS, El-Ahmary B, Al-Eisa RA. Mitigating Effect of Ginger against Oxidative Stress Induced by Atrazine Herbicides in Mice Liver and Kidney. J Biofertil Biopestici. 2011; 2:107.

Shah ZA, Vohora SB. Antioxidant/restorative effects of calcined gold preparations used in Indian systems of medicine against global and focal models of ischaemia. Pharmacology & toxicology. 2002; 90(5):254-259.

Mukherjee PK. Exploring botanicals in Indian system of medicine—regulatory perspectives. Clinical Research and Regulatory Affairs. 2003; 20(3):249-264.

Darbar S, Ghosh B, Chattopadhyay SP, Ghosh, B. Antioxidant and hepatoprotective activity of Livina, a polyherbal liquid formulation. Asian J Chem. 2009; 21(2):1495-1499.

Cederbaum AI, Lu Y, Wu D. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol, 2009; 83(6): 519-548.

Darbar S and Chattopadhyay SP. Single Dose Acute Oral Toxicity of Livina, a Polyherbal Formulation in Mice Model. European Journal of Pharmaceutical and Medical Research. 2018; 5(2): 492-495.

Darbar S and Chattopadhyay SP. Preliminary Single Dose Toxicological Investigation of Livina Capsule in Mice Model. International Journal of Research in Pharmacy and Pharmaceutical Sciences. 2020; 5(3): 26-28.

Darbar S, Chattopadhyay SP, Ghosh B, Chakraborty, MR. Effect of a polyherbal liquid formulation on aceclofenac induced gastric mucosal damage in albino wistar rats. J Pharm Res. 2008; 7(2):62-65.

Darbar S, Saha S, Chattopadhyay S, Chattapadhyay A. Anti-Stress Activity (in-vivo) of Multi Herbal Capsule-Trasina® in Experimental Murine Model. Asian Journal of Pharmaceutical Research and Development. 2020; 8(5):52-58.

Bhattacharya SK, Muruganandam AV. Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacology Biochemistry and Behavior. 2003; 75(3):547-555.

Darbar S, Chattopadhyay SP. Assessment of Acute Oral Toxicity Study of Trasina®, an Ayurvedic Herbal Formulation on Experimental Models. J. Pharm. Med. Res. 2019; 4(1): 84–86.

Darbar S, Saha S, K. Pramanik K, Chattopadhyay AK, Preliminary acute oral toxicity study of newly developed herbal formulation, World J Pharm Res. 2018; 7(5): 924-930.

Kashyap M, Hynd B, Robinson K. A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay. J Lipid Res. 1980; 21:491-505.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidase in animal tissue by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-358.

Beutler E. Red Cell Metabolism, a Manual of Biochemical Methods (third ed) Grune and Startton, 1984; New York p 133.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247: 3170-3175.

Beutler E, Olga D, Kelly M. Improved method for determination of blood glutathione. From the department of medicine, city of hope medical centre, J Lab Clin Med. 1963; 61: 882-888

Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite

Pharmacology. 1974; 11(3):151-169.

Hsu, J.Y.; Lin, H.H.; Hsu, C.C.; Chen, B.C. Chen, J.H. Aqueous Extract of Pepino (Solanum muriactum Ait) Leaves ameliorate lipid accumulation and oxidative stress in alcoholic fatty liver disease. Nutrients. 2018; 10: 931.

Milat, A.M.; Mudni´c, I.; Grkovi´c, I.; Kljuˇcevi´c, N.; Grga, M.; Jerˇci´c, I.; Juri´c, D.; Ivankovi´c, D.; Benzon, B.; Boban, M. E_ects of white wine consumption on weight in rats: Do polyphenols matter? Oxid. Med. Cell Longev 2017; 1: 1-7.

Lieber, C.S. Relationships between nutrition, alcohol use and liver disease. Alcohol Res Health. 2003; 27: 220–231.

Feinman, L. Lieber, C.S. Nutrition and diet in alcoholism. In Modern Nutrition in Health and Disease, 9th ed.; Shils, M.E., Olson, J.A., Shike, M., Ross, A.C., Eds.;Williams &Wilkins: Baltimore, MD, USA, 1998; pp. 1523–1542.

Zeyuan D, Bingyin T, Xiaolin L, Jinming H, Yifeng C. Effect of green tea and black tea on the blood glucose, the blood triglycerides and antioxidation in aged rats. J Agric Food Chem. 1998; 46:3875-3878.

Zararsiz I, Sarsilmaz M, Tas U, Kus I, Meydan S, Ozan E. Protective effect of melatonin against formaldehyde- induced kidney damage in rats. Toxicol Ind Health. 2007; 23:573-579.

Winterbourn CC. Concerted antioxidant activity of glutathione and superoxide dismutase. In: Packer L, Fuchs J (ed) Biothiols in health and disease. Marcel Dekker Inc, New York; 1995, pp 117-34.

Abuja PM, Albertini R. Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins. Clinica chimica acta. 2001; 306(1-2):1-7.




How to Cite

Darbar, S., Saha, S., & Chatopadhyay, A. (2021). Ethanol Intoxicated Hepatic Oxidative Stress Mitigated by Poly-Herbal Formulation – Trasina® in Murine Model . Innoriginal: International Journal of Sciences, 8(3). Retrieved from