Mitigating Effect of Herbal Medicine-Livina<sup>®</sup> upon Serum, Liver and Kidney Against Ethanol Induced Oxidative Stress in Mice
Abstract
Background: The aim and objective of the study of this study were to evaluate the possible ameliorative effect of herbal medicine (Livina®) on serum, liver, and kidney antioxidant enzymes activities in ethanol intoxicated organ dysfunctions in mice.Methods: Thirty-two Swiss albino adult male mice were randomly divided into four groups; Group-I as control, Group-IIorally treated with ethanol (50% v/v), Group-III three pre-treated with herbal medicine (Livina®) with ethanol (50% v/v), and Group-IV only treated with herbal medicine (Livina®) without ethanol daily. After six weeks the animals were euthanized and livers and kidneys were immediately removed and used fresh or kept frozen until analysis. Before the mice were killed blood samples were also collected to measure superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione –S transferees (GST) activities in sera.Results: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione –S transferees (GST) activities in serum, liver, and kidney were significantly reduced in the ethanol intoxicated mice than in the controls. Treatment with herbal medicine (Livina®) upon ethanol intoxication significantly elevated all antioxidant activities serum, liver, and kidney.Conclusions: In the present study our results predict that treatment with herbal medicine (Livina®) might be a potent antioxidant that exerts beneficial effects on both superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione –S transferees (GST) activities in ethanol intoxicated mice and inhibit organ damage.Keywords: Reactive oxygen species, antioxidant enzymes activity, Oxidative stress, Herbal formulation, Ethanol, Livina®, Swiss albino mice.References
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