INSILICO DOCKING ANAYSIS OF DELONIX ELATA LEAF EXTRACTS TO INHIBIT CERVICAL CANCER HPV16 IN ALBINO WISTAR RAT

Abstract

Cervical cancer is one of the most common forms of cancer diagnosed and causes death among women worldwide. Drug discovery from the medicinal plants has played an important role in the treatment of most human ailments and indeed. Delonix elata belongs to the family of caesalpinoideae, having promising medicinal property so; we investigated the anti-cancer potential of the leaf extract of D. elata Linn on Ehrlich Ascitis Carcinoma (EAC) tumor model. Tumor was induced in rat by intraperitoneal injection of EAC cells (1 ×106 cells/rat). D. elata was administered to the experimental animals at the dose levels of 250, 500 and 1000 mg/kg/day after 24 hours of tumor inoculation. The anti-tumor effect of D. elata was evaluated by assessing the body weight, survival time and haematological parameters (  ). Oral administration of D. elata extended the survival time of the EAC bearing rat. The D. elata normalized the levels of the hematological parameters in a dose dependent manner in EAC bearing rat when compared to control. The results were comparable to that of the result obtained from the animals treated with the standard drug 5-flurouracil (20mg/kg/b.w). In this study, we used petroleum ether leaf extract of D.elata and also isolated bioactive compounds namely 2-(4-methoxyphenyl)imino)-4-methyl-1,3-thiazolan-4-yl)methanol,5-(p-aminophenyl)-4-(o-tolyl)-2 thiazolamine and Dodecanoic acid, 2,3-bis(acetyloxy)  propylester by GC-MS. The 3-D crystal structure of the HPV16 E6 oncoprotein (PDB-ID: 2FK4) responsible for cervical cancer was retrieved from the Protein Data Bank (PDB). The compound 5-(p-aminophenyl)-4-(o-tolyl)-2 thiazolamine shows best docking energy values compare than other compound -10.7013 kcal/mol. Thus present study revealed that D. elata possessed 5-(p-aminophenyl)-4-(o-tolyl)-2 thiazolamine has significant antitumor activity.