ANTIOXIDANT EFFECTS OF METHANOLIC STEM-BARK EXTRACT OF PTEROCARPUS ERINACEUS ON GASTRIC ACID SECRETION
Abstract
Objective: The antioxidant of methanolic stem-bark extract of Pterocarpus erinaceus on indomethacin-induced ulcer in albino rats was studied. Methods: Phytochemicals in the bark extract were qualitatively and quantitatively determined. Antioxidant status of the extract was determined using DPPH, FRAP and TBARS assays. The activities of glutathione reductase, superoxide dismutase and catalase effects were also determined along with total protein concentration in the stomach tissue homogenate. Thirty five (35) adult male rats were randomly divided into seven groups (Group A-G) of five rats each. The in-vitro antioxidant status of the extract was also determined. Indomethacin was orally administered as a single dose of 30 mg/kg/b.w to induce ulcer in the rats. The rats were fasted for 24 hrs, on the last day of the experiment before the animals were sacrificed. Macroscopic examinations of the stomach, quantification of gastric juice, homogenization of stomach were also carried out. The stomach homogenate of treated rats was analyzed for total protein (TP), lipid peroxidation and the antioxidant enzymes glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) activities. Results: Indomethacin treatment caused significant increases (p˂ 0.05) in ulcer index. The administration of the methanolic stem-bark extract of Pterocarpus erinaceus, rats previously treated with indomethacin showed significant reduction in ulcer index, volumes of gastric juice, free and total acidity, pepsin activity and lipid peroxidation in the extract treated rats compared with the group treated with indomethacin. Treatments with P. erinaceus extract showed significant dose dependent elevation (p˂ 0.05) of pH of gastric juice, total protein, GR, SOD and CAT in the treated animals. Conclusion: These results demonstrate that the plant extract may have antioxidant potentials suggesting that P. erinaceus stem bark extract at doses used have the ability to reduce gastric acid secretion and healing of the ulcer.Keywords: Antioxidant, Effect, Albino rats, Ulcer, Stem-bark Extracts, phytochemical and IndomethacinReferences
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