Mathematical Processing for Simultaneous Spectrophotometric Determination of Cyclopentolate and Phenylephrine in Their Binary Mixture
Abstract
Introduction: Development and validation of three simple, accurate, selective and and low cost spectrophotometric methods for simultaneous determination of cyclopentolate hydrochloride (CLO) and phenylephrine hydrochloride (PHE) in their binary mixture without need of any pre-separation step or sophisticated instruments. These methods are (I) area under the curve method using two ranges of wavelengths (255 - 260) and (271 - 275) nm.  (II) Q-analysis method using two wavelengths 281.2 nm (iso-absorptive point) and 291 nm. (III) bivariate method using two wavelengths 255 and 270 nm choosen according to Kaiser method to calculate the sensitivity matrices of different wavelengths. Results and discussion: Calibration graphs were established in the range of 10-130 µg/ml for both drugs with good correlation coefficients. Conclusions: All the proposed methods were validated and successfully applied for simultaneous determination of cyclopentolate hydrochloride (CLO) and phenylephrine hydrochloride (PHE) in their bulk powder and pharmaceutical preparation Cyclophrine® eye drops. The obtained results were statistically compared with those of the reported method by applying student’s t-test and F-test at 95 % confidence level and no significant difference was observed regarding accuracy and precision.References
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