DEVELOPMENT AND VALIDATION OF A NEW RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CEFIXIME AND LINEZOLID IN TABLET DOSAGE FORMS

kiran jyothi

Abstract


A simple, precise, stability indicating new RP-HPLC method was developed and validated for the Simultaneous determination of Cefixime and Linezolid. In this method separation of liquid was done by using column Inertsil ODSC18, 150X4.6mm, 5µ with mobile phase of 0.1M Sodium Dihydrogen Ortho Phosphate (pH 4.7) and Acetonitrile were mixed in the ratio of 60:40 ratio. The detection wavelength was found to be 272 nm with a flow rate of 1ml/minute and temperature of 300C. Retention time of Cefixime was 3.161 minutes and Linezolide was 4.774 minutes respectively. The proposed method was validated as per standard guidelines. In the range of 8µg to 45µg the linearity of Cefixime shows a co-relation coefficient of 0.9991 and Linezolide was 24 to144 µg 0.9991 respectively. System precision was found % RSD 0.31 and 0.27, whereas method precision was found % RSD to be 0.24 and 0.55 of Cefixime and Linezolide respectively. The % mean recovery of Cefixime and Linezolide was found to be 99.87 and 99.77% respectively. The method was found to be robust even by changing in the flow rate 0.1ml and temperature change of ±5 condition .The developed method can be successfully employed for the routine analysis of Cefixime and Linezolide in pharmaceutical dosage forms.


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References


Indian pharmacopeia, Govt. of India, Ministry of Health and family welfare, published by the Indian Pharmacopoeial commission, 2, 2010, 1590-1592.

The United State Pharmacopoeia, 28th ed, The United States Pharmacopoeial Convention Inc., Rockville, 2005; 1413-15,1834.

Rang HP, Dale MM, Ritter JM and Flower RJ, Book of Pharmacology, 6thed, Elsevier’s Publication, 2006, 666-672.

Patel NS, Tandel FB, Patel YD, Thakkar KB. Development and validation of stability-indicating HPLC method for simultaneous estimation of cefixime and linezolid. Indian J Pharm Sci 2014; 76:535-40

Satish A. Patel and Jinalben V Patel. Rp-HPLC method for simultaneous estimation of cefixime trihydrate and linezolid in tablet dosage form. IJPCBS 2013, 3(2), 372-379.

Dolly t.Gadhiya, Hina l. Bagada.Simultaneous equation method for the estimation of cefixime trihydrate and linezolid in their combined tablet dosage form by UV-visible spectrophotometry.International Bulletin of Drug Research.,3(5): 29-38, 2013.

Patel DP, Goswami K, Patel M.Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Cefixime and Linezolid in Combined Dosage Form.Int J Pharm Res Scholars.V-1, I-4, 2012.

Dharuman J, RavichandranV, PillaiKM, Spectrophotometric method for estimation of linezolid in tablets, Indian J. PharmSci, 2004;235-236.

Virypaxappa BS, Shivaprasad KH, Latha MS, A Simple Method for Spectrophotometric Determination of Cefixime in Pharmaceuticals; IntJ.Chem Res; 2010; 2(1):23-30.

Dhoka MV, VaidyaPravin D, PandeAmol V, AroraAseem A, Development and Validation of Analytical Method for Estimation of Cefixime in Swab Samples, IntJ.ChemTech Res, 2011;2(4):1918-1923.

SatyanarayanaBattu, VasudevPottbathini. A new stability indicating hplc method for simultaneous estimation of cefixime and linezolid in pharmaceutical dosage form and plasma sample. Int J Pharm 2014; 4(1):321-329.

Dishathakkar, Rajshreemashru. Simultaneous estimation of cefiximetrihydrate and linezolid in combine pharmaceutical formulation.Int J Pharm PharmSci, Vol 5, Issue 1, 76-81,2013.

ICH guidelines, Validation of Analytical Procedures: Text and Methodology, Q2(R1) Nov 2005.


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Innoriginal: International Journal of Sciences (ISSN: 2349-7041)

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