Ahmed Madkour


Four simple, accurate, selective, reproducible and sensitive spectrophotometric methods, each method based on selection of two wavelengths, have been developed and validated for the determination of oxfendazole in the presence of its alkali-induced degradation product without preliminary separation. These methods include (A) dual wavelength method at 291.6 and 304.9 nm, (B) Q-analysis method at 275.5 and 292 nm, (C) simultaneous equation method at 292 and 299 nm and (D) absorbance ratio method at 275.5 and 292 nm. These methods were validated and successfully applied for determination of oxfendazole in Unifendazole® suspension. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision. 

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- Windholz M. (1983). The Merck Index: an encyclopedia of Chemicals, Drugs and biologicals, tenth ed., Merck & co., Inc., Rahway, NJ, USA.

- Reynolds JEF. (1996), Martindale, The Extra Pharmacopia, 31st edition, The Royal Pharmaceutical Society, London.

-United States Pharmacopoeia 34th ed, National formulary 29, Official monographs, 2011.

- Rajendraprasad N, Basavaiah K. (2016). Potentiometric determination of piroxicam and oxfendazole in pharmaceuticals. Current Chemistry Letters; 5(1):33-46.

- Nerenberg C, Runkel RA, Matin S B. (1978). Radioimmunoassay of oxfendazole in bovine, equine, or canine plasma or serum. J pharm sci; 67(11): 1553-1557.

- Rajendraprasad N, Basavaiah K. (2016). Uv/Visible Spectroscopic Quantification Of Veterinary Anthelmintic Drug Oxfendazole In Pharmaceuticals. J Applied Spectro; 83(3):392-397.

- Tsina IW, Matin SB.(1981). Determination of Oxfendazole in Cow Milk by Reversed Phase HighPerformance Liquid Chromatography. J Pharm Sci; 70 (8):858-860.

- Su SC, Chou HH, Chang PC, Liu CH, Chou SS, (2004). Simultaneous Determination of Febantel, Fenbendazole,Oxfendazole and Oxfendazole Sulfone in Livestock by Matrix Solid Phase Dispersion ExtractionTechnique and HPLC. J Food and Drug Analysis; 12 (3): 244-253.

- Blanchflower WJ, Cannavan A, Kennedy DG, (1994). Determination of fenbendazole and oxfendazole in liver and muscle using liquid chromatography mass spectrometry. Analyst; 119 (6):1325-8.

- Shah G, Bradley D, Shek E, (1984). Liquid chromatographic determination of oxfendazole in swine feeds. J Assoc Off Anal Chem; 67(4):707-14.

- Fleitman J, Neu D, Benjamin E, (1986). Analysis of pharmaceutical dosage forms for oxfendazole: II. Simultaneous liquid chromatographic determination of oxfendazole and trichlorfon in equine paste. J Assoc Off Anal Chem; 69(1):24-8.

- Khan AR, Akhtar MJ, Mahmood R, Ahmed SM, Malook S, Iqbal M.(2000). LC assay method for oxfendazole and oxyclozanide in pharmaceutical preparation. Journal of Pharm and Biomedical Anal; 22(1): 111-114.

- Kulik A, Bialecka W, Podolska M, Puchniarz BK, Mazurek A.(2011). HPLC Method For Identification And Quantification Of Benzimidazole Derivatives In Antiparasitic Drugs. Acta Poloniae Pharmaceutica -Drug Research; 68 (6):823-829.

- Tai SS, Cargile N, Barnes CJ.(1990). Determination of thiabendazole, 5 hydroxy thiabendazole, fenbendazole, and oxfendazole in milk. J Assoc Off Anal Chem; 73(3):368-373.

- Havlíková L, Brabcová I, Satinsky D, Solich P.(2012). Optimisation of an HPLC method for the simultaneous determination of pyrantel pamoate, praziquantel, fenbendazole, oxfendazole and butylhydroxyanisole using a phenyl stationary phase. Anal methods; 4(6):1592-1597•

¬- Nakos DS, Botsoglou NA, Psomas IE. (1994). Ion- Pair Isolation and Liquid Chromatographic Dmineteration of Albendazole, Oxfendazole, Oxibendazole, and Thiabendazole Residues in Milk. J Liquid Chromatography; 17(19):4145- 4155.

- Klausz G, Keller E, Sára Z, Körmöczy PS, Laczay P, Ary K, Sótonyi P, Róna K.(2015). Simultaneous determination of praziquantel, pyrantel embonate, febantel and its active metabolites, oxfendazole and fenbendazole, in dog plasma by liquid chromatography / mass spectrometry. Biomed. Chromatogr; 29: 1859–1865.

- Fernandes N, Nimdeo M S, Choudhar V P, Kulkarni R R, Pande V V, Nikalje A G (2008). Dual wavelength and simultaneous equation spectrophotometric methods for estimation of atenolol and indapamide in their combined dosage form. Int J Chem Sci; 6(1):29-35.

- Oza CK, Nijhawan R, Pandya MK, Vyas AJ, Patel AI (2012). Dual wavelength spectrophotometric method for the simultaneous determination of paracetamol and nabumetone in tablet dosage form. Asian J Pharm Anal; 2(4): 122-127.

- Bhusari KP, Khedkar PB, Dhole S, Banode VS (2009). Derivative and Q-analysis Spectrophotometric methods for estimation of hydrochlorothiazide and olmesartan medoxomil in tablets. Indian J Pharm Sci; 71(5): 505–508.

- Abdel-Wahab NS (2011), Spectrophotometric methods for simultaneous determination of carvedilol and hydrochlorothiazide in combined dosage form. Arabian J chem; 1016:10-15.

- Begum H, Rizwan S, Bin sayeed K, Fatima A (2015). Development and validation of stability indicating UV spectrophotometric Simultaneous equation method for determination of ezetimibe and Glimepiride in bulk drugs and marketed formulation. Int J Res and Development in Pharm and Life Sci; 4:1412-1421.

- Beckett AH, Stenlake JB (1997). Practical Pharmaceutical Chemistry, 4th Ed, Vol. II, CBS Publishers and Distributors, New Delhi. 284.

- Darwish HW, Hassan SA, Salem MY, El-Zeany BA (2013). Three different methods for determination of binary mixture of amLodipine and atorvastatin using dual wavelength spectrophotometry. Spectrochim Acta A; 104:70-76.

- ICH, Q2 (R1) (2005).Validation of Analytical Procedures, Proceedings of International Conference on Harmonization, Geneva.


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