FREE RADICAL SCAVENGING POTENTIAL AND ANTIOXIDANT ACTIVITY OF SILVER NANOPARTICLE COUPLED WITH ANDROGRAPHIS PANICULATA (AP-AG NP) AGAINST CARBON TETRACHLORIDE (CCL4) INDUCED TOXICITY IN MICE

Soumendra Darbar, Srimoyee Saha, Atiskumar Chattopadhyay

Abstract


Background: Nanomedicine is a branch of medicine that applies the knowledge and tools of nanotechnology to the prevention and treatment of life threatening disease. Therapeutic exposure to Silver Nanoparticle Coupled with Andrographis paniculata (AP-Ag NP) was assed against CCl4 induced oxidative stress in tissue level. Aims and Objectives: The present study aimed to evaluate the comparative efficacy of AP-Ag NP against Carbon tetrachloride (CCl4) induced oxidative stress. Materials and Methods: Male swiss albino mice were exposed to Carbon tetrachloride (CCl4) for 28 days and were co-administered orally with AP-Ag NP (50 mg/kg each) to evaluate effects on heme synthesis pathway, hepatic oxidative injury and antioxidant potential. Results: The results suggest a significantly elevated ROS, decreased blood and hepatic GSH levels, Superoxide dismutase and Catalase activities after Carbon tetrachloride (CCl4) exposure. Co-administration of Nano-antioxidants (AP-Ag NP) increased SOD, CAT and GSH levels and was also beneficial in the recovery of oxidative injury and restoring inhibited aminolevulinate dehydratase (ALAD) activity. Conclusion: Our results suggest that Silver Nanoparticle Coupled with Andrographis paniculata (AP-Ag NP) have the potential antioxidant effect in mice.

 

Keywords: AgNP; Andrographis paniculata; Protection; Free radicals; Lipid peroxidation.


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