THE PROTECTIVE EFFECT OF PANDANUS AMARYLLIFOLIUS LEAVES EXTRACT ON PARACETAMOL-INDUCED HEPATOTOXICITY

Yuandani ., Finni Riski Aulia Sagala

Abstract


ABSTRACT

 

Objective: This current study was carried out to investigate the hepatoprotective activity of Pandanus amaryllifolius on paracetamol-induced male rats.

 

Methods: The hepatoprotective activity of ethanol extract of P. amaryllifolius leaves was evaluated in paracetamol-induced male rats. The extract was administered orally at doses of 50, 100, 200 mg/kg bw for 7 days. On day 7, liver damage was induced by high dose of paracetamol (1.35g/kg bw). The levels of alanin aminotransferase (ALT) and aspartat aminotranferase (AST) were determined to evaluate the liver function.  Microscopic assessment of hepatic tissue section was also performed. The hepatoprotective effect of extract was compared to normal, positive and negative control.

 

Results: All doses of ethanol extract of P. amaryllifolius leaves prevented increase in serum level of ALT and AST as compared to negative control (P<0.05). However, only dose of 200 mg/kg bw displayed comparable hepatoprotective activity to normal and positive control (P>0.05). The result was supported by histological assessment which showed no liver damage at highest dose of P. amaryllifolius extract.

Conclusion: The result emphasize that ethanol extract of P. amaryllifolius leaves possesses hepatoprotective effect.

Keywords: P. amaryllifolius, ALT, AST, histopathology


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