DIFFERENT SPECTRAL DATA PROCESSING TECHNIQUES FOR DETERMINATION OF LEDIPASVIR AND SOFOSBUVIR IN THEIR PURE AND DOSAGE FORMS; A COMPARATIVE STUDY.

Abstract

Four techniques were described for simultaneous determination of sofosbuvir and ledipasvir in their pure and pharmaceutical dosage forms. The first and second methods describe the development of classical least squares (CLS) and principle component regression (PCR) chemometric models, the third and fourth methods describe the development and validation of spectrophotometric methods namely; simultaneous equation and amplitude modulation. The four methods were successfully applied to quantify sofosbuvir and ledipasvir in laboratory prepared mixtures and real market sample. The investigated methods were found to be accurate, precise and can resolve the overlapped spectra of the mixture without any preliminary separation steps.